active rhoa levels Search Results


rhoagtp levels  (Cytoskeleton Inc)
95
Cytoskeleton Inc rhoagtp levels
Rhoagtp Levels, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rho activation assay kit  (Millipore)
90
Millipore rho activation assay kit
Rho Activation Assay Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rho assay reagent  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc rho assay reagent
Rho Assay Reagent, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rho assay reagent/product/Upstate Biotechnology Inc
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rhoa  (Cytoskeleton Inc)
97
Cytoskeleton Inc rhoa
<t>RhoA,</t> <t>Rac1,</t> and Cdc42 activity is decreased in DU-145 prostate cancer cells but not RWPE-1 normal prostate epithelial cells. (A, C, E) Treatment with 1 mM BA or 1 mM PBA of DU-145 cells serum starved overnight and plated on fibronectin for 15 min in the presence of 10% FBS caused a significant inhibition of each protein compared to untreated cells (*). One millimolar PBA caused a significant decrease in protein activity compared to untreated (*) and BA treated cells (**). (B, D, F) Neither compound inhibited RhoA, Rac1, or Cdc42 activity in the normal cells. Results expressed as mean ± SEM for three replicates.
Rhoa, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rhoa/product/Cytoskeleton Inc
Average 97 stars, based on 1 article reviews
rhoa - by Bioz Stars, 2026-03
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rho activation assay kit  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc rho activation assay kit
<t>RhoA,</t> <t>Rac1,</t> and Cdc42 activity is decreased in DU-145 prostate cancer cells but not RWPE-1 normal prostate epithelial cells. (A, C, E) Treatment with 1 mM BA or 1 mM PBA of DU-145 cells serum starved overnight and plated on fibronectin for 15 min in the presence of 10% FBS caused a significant inhibition of each protein compared to untreated cells (*). One millimolar PBA caused a significant decrease in protein activity compared to untreated (*) and BA treated cells (**). (B, D, F) Neither compound inhibited RhoA, Rac1, or Cdc42 activity in the normal cells. Results expressed as mean ± SEM for three replicates.
Rho Activation Assay Kit, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rho activation assay kit/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
rho activation assay kit - by Bioz Stars, 2026-03
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rhoa levels  (Cytoskeleton Inc)
95
Cytoskeleton Inc rhoa levels
<t>RhoA,</t> <t>Rac1,</t> and Cdc42 activity is decreased in DU-145 prostate cancer cells but not RWPE-1 normal prostate epithelial cells. (A, C, E) Treatment with 1 mM BA or 1 mM PBA of DU-145 cells serum starved overnight and plated on fibronectin for 15 min in the presence of 10% FBS caused a significant inhibition of each protein compared to untreated cells (*). One millimolar PBA caused a significant decrease in protein activity compared to untreated (*) and BA treated cells (**). (B, D, F) Neither compound inhibited RhoA, Rac1, or Cdc42 activity in the normal cells. Results expressed as mean ± SEM for three replicates.
Rhoa Levels, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rhoa levels - by Bioz Stars, 2026-03
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rhoa protein  (Cytoskeleton Inc)
93
Cytoskeleton Inc rhoa protein
<t>RhoA,</t> <t>Rac1,</t> and Cdc42 activity is decreased in DU-145 prostate cancer cells but not RWPE-1 normal prostate epithelial cells. (A, C, E) Treatment with 1 mM BA or 1 mM PBA of DU-145 cells serum starved overnight and plated on fibronectin for 15 min in the presence of 10% FBS caused a significant inhibition of each protein compared to untreated cells (*). One millimolar PBA caused a significant decrease in protein activity compared to untreated (*) and BA treated cells (**). (B, D, F) Neither compound inhibited RhoA, Rac1, or Cdc42 activity in the normal cells. Results expressed as mean ± SEM for three replicates.
Rhoa Protein, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rhoa protein - by Bioz Stars, 2026-03
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rac1 gtp  (Cytoskeleton Inc)
95
Cytoskeleton Inc rac1 gtp
<t>RhoA,</t> <t>Rac1,</t> and Cdc42 activity is decreased in DU-145 prostate cancer cells but not RWPE-1 normal prostate epithelial cells. (A, C, E) Treatment with 1 mM BA or 1 mM PBA of DU-145 cells serum starved overnight and plated on fibronectin for 15 min in the presence of 10% FBS caused a significant inhibition of each protein compared to untreated cells (*). One millimolar PBA caused a significant decrease in protein activity compared to untreated (*) and BA treated cells (**). (B, D, F) Neither compound inhibited RhoA, Rac1, or Cdc42 activity in the normal cells. Results expressed as mean ± SEM for three replicates.
Rac1 Gtp, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rhoa gtpases  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc rhoa gtpases
FIGURE 6 | Radiofrequency electromagnetic fields (RF-EMF) exposure decreased CREB phosphorylation through EPHA5. (A) RF-EMF exposure inhibited CREB phosphorylation in NSC-derived cells. **p < 0.01 by Student t-test. (B) RF-EMF exposure reduced the phosphorylation of CREB in Neuro-2A cells. **p < 0.01 by Student t-test. (C) EPHA5 silencing decreased CREB phosphorylation in NSC-derived cells. **p < 0.01 by Student t-test. (D) EPHA5 recombinant protein treatment rescued CREB phosphorylation after RF-EMF exposure in NSC-derived cells. *p < 0.05, and **p < 0.01 by Student t-test. (E) RF-EMF exposure increased the GTP-bound <t>RhoA</t> level. (F) EPHA5 silencing increased the GTP-bound RhoA level. (G) EPHA5 recombinant protein treatment reversed the GTP-bound RhoA level after RF-EMF exposure.
Rhoa Gtpases, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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total rhoa  (Cytoskeleton Inc)
95
Cytoskeleton Inc total rhoa
FIGURE 6 | Radiofrequency electromagnetic fields (RF-EMF) exposure decreased CREB phosphorylation through EPHA5. (A) RF-EMF exposure inhibited CREB phosphorylation in NSC-derived cells. **p < 0.01 by Student t-test. (B) RF-EMF exposure reduced the phosphorylation of CREB in Neuro-2A cells. **p < 0.01 by Student t-test. (C) EPHA5 silencing decreased CREB phosphorylation in NSC-derived cells. **p < 0.01 by Student t-test. (D) EPHA5 recombinant protein treatment rescued CREB phosphorylation after RF-EMF exposure in NSC-derived cells. *p < 0.05, and **p < 0.01 by Student t-test. (E) RF-EMF exposure increased the GTP-bound <t>RhoA</t> level. (F) EPHA5 silencing increased the GTP-bound RhoA level. (G) EPHA5 recombinant protein treatment reversed the GTP-bound RhoA level after RF-EMF exposure.
Total Rhoa, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rho antibody  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc rho antibody
FIGURE 6 | Radiofrequency electromagnetic fields (RF-EMF) exposure decreased CREB phosphorylation through EPHA5. (A) RF-EMF exposure inhibited CREB phosphorylation in NSC-derived cells. **p < 0.01 by Student t-test. (B) RF-EMF exposure reduced the phosphorylation of CREB in Neuro-2A cells. **p < 0.01 by Student t-test. (C) EPHA5 silencing decreased CREB phosphorylation in NSC-derived cells. **p < 0.01 by Student t-test. (D) EPHA5 recombinant protein treatment rescued CREB phosphorylation after RF-EMF exposure in NSC-derived cells. *p < 0.05, and **p < 0.01 by Student t-test. (E) RF-EMF exposure increased the GTP-bound <t>RhoA</t> level. (F) EPHA5 silencing increased the GTP-bound RhoA level. (G) EPHA5 recombinant protein treatment reversed the GTP-bound RhoA level after RF-EMF exposure.
Rho Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rho/rock activation  (Amano Inc)
90
Amano Inc rho/rock activation
FIGURE 6 | Radiofrequency electromagnetic fields (RF-EMF) exposure decreased CREB phosphorylation through EPHA5. (A) RF-EMF exposure inhibited CREB phosphorylation in NSC-derived cells. **p < 0.01 by Student t-test. (B) RF-EMF exposure reduced the phosphorylation of CREB in Neuro-2A cells. **p < 0.01 by Student t-test. (C) EPHA5 silencing decreased CREB phosphorylation in NSC-derived cells. **p < 0.01 by Student t-test. (D) EPHA5 recombinant protein treatment rescued CREB phosphorylation after RF-EMF exposure in NSC-derived cells. *p < 0.05, and **p < 0.01 by Student t-test. (E) RF-EMF exposure increased the GTP-bound <t>RhoA</t> level. (F) EPHA5 silencing increased the GTP-bound RhoA level. (G) EPHA5 recombinant protein treatment reversed the GTP-bound RhoA level after RF-EMF exposure.
Rho/Rock Activation, supplied by Amano Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


RhoA, Rac1, and Cdc42 activity is decreased in DU-145 prostate cancer cells but not RWPE-1 normal prostate epithelial cells. (A, C, E) Treatment with 1 mM BA or 1 mM PBA of DU-145 cells serum starved overnight and plated on fibronectin for 15 min in the presence of 10% FBS caused a significant inhibition of each protein compared to untreated cells (*). One millimolar PBA caused a significant decrease in protein activity compared to untreated (*) and BA treated cells (**). (B, D, F) Neither compound inhibited RhoA, Rac1, or Cdc42 activity in the normal cells. Results expressed as mean ± SEM for three replicates.

Journal: Cell Adhesion & Migration

Article Title: Phenylboronic acid is a more potent inhibitor than boric acid of key signaling networks involved in cancer cell migration

doi: 10.4161/cam.5.5.18162

Figure Lengend Snippet: RhoA, Rac1, and Cdc42 activity is decreased in DU-145 prostate cancer cells but not RWPE-1 normal prostate epithelial cells. (A, C, E) Treatment with 1 mM BA or 1 mM PBA of DU-145 cells serum starved overnight and plated on fibronectin for 15 min in the presence of 10% FBS caused a significant inhibition of each protein compared to untreated cells (*). One millimolar PBA caused a significant decrease in protein activity compared to untreated (*) and BA treated cells (**). (B, D, F) Neither compound inhibited RhoA, Rac1, or Cdc42 activity in the normal cells. Results expressed as mean ± SEM for three replicates.

Article Snippet: The enzyme-linked immunosorbent assay (ELISA)-based G-LISA absorbance based kits (Cytoskeleton) were used to determine endogenously activated (GTP bound) levels RhoA (#BK124), Rac1 (#BK035), and Cdc42 (#CN02) in DU145 and RWPE-1 cells according to the manufacturer's instructions.

Techniques: Activity Assay, Inhibition

FIGURE 6 | Radiofrequency electromagnetic fields (RF-EMF) exposure decreased CREB phosphorylation through EPHA5. (A) RF-EMF exposure inhibited CREB phosphorylation in NSC-derived cells. **p < 0.01 by Student t-test. (B) RF-EMF exposure reduced the phosphorylation of CREB in Neuro-2A cells. **p < 0.01 by Student t-test. (C) EPHA5 silencing decreased CREB phosphorylation in NSC-derived cells. **p < 0.01 by Student t-test. (D) EPHA5 recombinant protein treatment rescued CREB phosphorylation after RF-EMF exposure in NSC-derived cells. *p < 0.05, and **p < 0.01 by Student t-test. (E) RF-EMF exposure increased the GTP-bound RhoA level. (F) EPHA5 silencing increased the GTP-bound RhoA level. (G) EPHA5 recombinant protein treatment reversed the GTP-bound RhoA level after RF-EMF exposure.

Journal: Frontiers in cell and developmental biology

Article Title: 1800 MHz Radiofrequency Electromagnetic Field Impairs Neurite Outgrowth Through Inhibiting EPHA5 Signaling.

doi: 10.3389/fcell.2021.657623

Figure Lengend Snippet: FIGURE 6 | Radiofrequency electromagnetic fields (RF-EMF) exposure decreased CREB phosphorylation through EPHA5. (A) RF-EMF exposure inhibited CREB phosphorylation in NSC-derived cells. **p < 0.01 by Student t-test. (B) RF-EMF exposure reduced the phosphorylation of CREB in Neuro-2A cells. **p < 0.01 by Student t-test. (C) EPHA5 silencing decreased CREB phosphorylation in NSC-derived cells. **p < 0.01 by Student t-test. (D) EPHA5 recombinant protein treatment rescued CREB phosphorylation after RF-EMF exposure in NSC-derived cells. *p < 0.05, and **p < 0.01 by Student t-test. (E) RF-EMF exposure increased the GTP-bound RhoA level. (F) EPHA5 silencing increased the GTP-bound RhoA level. (G) EPHA5 recombinant protein treatment reversed the GTP-bound RhoA level after RF-EMF exposure.

Article Snippet: The level of activated RhoA GTPases in cells after RF-EMF exposure was determined with an active Rho detection kit purchased from Cell Signaling Technology according to previous work (Sin et al., 2020).

Techniques: Phospho-proteomics, Derivative Assay, Recombinant

FIGURE 7 | Forskolin (FSK) treatment rescued neurite outgrowth in RF-EMF exposed cells. (A,B) FSK treatment antagonized the inhibitory effects of RF-EMF exposure on neurite outgrowth in NSC-derived cells. (C,D) FSK treatment rescued neurite outgrowth in Neuro-2A cells after RF-EMF exposure. *p < 0.05, and **p < 0.01, one-way ANOVA followed by Bonferroni post-tests. (E,F) FSK treatment rescued the protein expression of DCX. **p < 0.01, One-way ANOVA followed by Bonferroni post-tests. (G) FSK treatment reversed the GTP-bound RhoA level after RF-EMF exposure.

Journal: Frontiers in cell and developmental biology

Article Title: 1800 MHz Radiofrequency Electromagnetic Field Impairs Neurite Outgrowth Through Inhibiting EPHA5 Signaling.

doi: 10.3389/fcell.2021.657623

Figure Lengend Snippet: FIGURE 7 | Forskolin (FSK) treatment rescued neurite outgrowth in RF-EMF exposed cells. (A,B) FSK treatment antagonized the inhibitory effects of RF-EMF exposure on neurite outgrowth in NSC-derived cells. (C,D) FSK treatment rescued neurite outgrowth in Neuro-2A cells after RF-EMF exposure. *p < 0.05, and **p < 0.01, one-way ANOVA followed by Bonferroni post-tests. (E,F) FSK treatment rescued the protein expression of DCX. **p < 0.01, One-way ANOVA followed by Bonferroni post-tests. (G) FSK treatment reversed the GTP-bound RhoA level after RF-EMF exposure.

Article Snippet: The level of activated RhoA GTPases in cells after RF-EMF exposure was determined with an active Rho detection kit purchased from Cell Signaling Technology according to previous work (Sin et al., 2020).

Techniques: Derivative Assay, Expressing